抗体 单克隆抗-FLAG®

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in association with other tags. The small size of the FLAG tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function. The N-terminal FLAG peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage catalyzed by Cu²⁺ ions of the C-terminal FLAG peptide from a fusion protein has been reported. A sequence motif with five out of eight amino acid residues identical to the FLAG peptide is found in both rat and mouse Mg²⁺dependent protein b-phosphatase, as well as in the human and bovine enzyme.

兔抗-FLAG抗体源自SIG1-25杂交瘤细胞。 杂交瘤细胞是通过接种偶联KLH的FLAG^®肽链序列的兔骨髓瘤细胞和脾细胞融合获得。